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KMID : 0615720140310040045
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Jouranl of Korean Acupuncture and Moxibustion society
2014 Volume.31 No. 4 p.45 ~ p.55
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Comparison of the Ingredient Quantities, and Antioxidant and Anti-inflammatory Activities of Hwangryunhaedok Decoction Pharmacopuncture by Preparation Type
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Lee Jin-Ho
Kim Min-Jeong
Lee Jae-Woong
Kim Me-Riong
Lee In-Hee
Kim Eun-Jee
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Abstract
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Objectives: The main aim of this study was to assess the comparative efficiency of two preparation types of Hwangryunhaedok decoction(HRHD-D) using distilled and mixed extraction by measuring the index components and indicators of antioxidant and anti-inflammatory effects.
Methods: The antioxidant activity was assessed by comparing distilled and mixed extractions of HRHD-D using an ELISA reader. The anti-inflammatory effect was determined by measuring NO amounts in RAW 264.7 cells. The contents were analyzed with high performance liquid chromatography-diode array detector(HPLC-DAD).
Results: The electron donating ability of mixed and distilled extractions obtained with 500 ppm DPPH(1,1-diphenyl-2-picrylhydrazyl assay) solution were 57.8 % and 4.2 %, respectively. The total phenolic content of mixed extraction was 6.9 times that of distilled extraction and total flavonoid content was 51.5 times higher. The anti-inflammatory effect was assessed by NO measurement, and was found to increase significantly dependent on concentration in all mixed extract concentrations(25, 50, 100, 200, 400 μg/mL), but the difference in distilled extraction by concentration was only significant at 200 and 400 μg/mL. The HPLC analysis results of mixed extract of HRHD-D showed detection of all four main active constituents of HRHD-D. However, they were not detected in the distilled extract of HRHD-D.
Conclusions: Mixed extraction with distillation added to decoction of HRHD-D showed better efficacy in antioxidant and anti-inflammatory effects, and ingredient quantities compared to distilled extraction. Further stability and clinical efficacy studies for standardization of mixed extractions are required.
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KEYWORD
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Hwangryunhaedok decoctiom, Pharmacopuncture, Antioxidant activity, Anti-inflammatory activity, HPLC.
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